Pinhole Size Lookup Table
The input values (objective magnification, machine number)
are recorde in the image files.
The output is the size of the pinhole backprojected
into the sample plane (in nm).
The latter value is used as input for the Huygens
deconvolution system.
Magnification 100x
Magnification 63x
 |
|
|
|
|
| Objective Magnification:
100x |
| Value |
Leica TCS (nm) |
Zeiss LSM 310 (nm) |
Zeiss LSM 410 (nm) |
Zeiss LSM 510 (nm)
= digital value / (mag*160/48) |
|
10
|
47
|
100
|
100
|
30
|
|
20
|
70
|
200
|
199
|
60
|
|
30
|
93
|
300
|
299
|
90
|
|
40
|
116
|
400
|
398
|
120
|
|
50
|
139
|
500
|
498
|
150
|
|
60
|
162
|
600
|
597
|
180
|
|
70
|
185
|
700
|
697
|
210
|
|
80
|
208
|
800
|
796
|
240
|
|
90
|
232
|
900
|
896
|
270
|
|
100
|
255
|
1000
|
996
|
300
|
|
110
|
278
|
1100
|
1095
|
330
|
|
120
|
301
|
1200
|
1195
|
360
|
|
130
|
324
|
1300
|
1294
|
390
|
|
140
|
347
|
1400
|
1394
|
420
|
|
150
|
370
|
1500
|
1493
|
450
|
|
160
|
393
|
1600
|
1593
|
480
|
|
170
|
416
|
1700
|
1692
|
510
|
|
180
|
439
|
1800
|
1792
|
540
|
|
190
|
462
|
1900
|
1891
|
570
|
|
200
|
485
|
2000
|
1991
|
600
|
|
210
|
508
|
2100
|
2091
|
630
|
|
220
|
531
|
2200
|
2190
|
660
|
|
230
|
554
|
2300
|
2290
|
690
|
|
240
|
577
|
2400
|
2389
|
720
|
|
250
|
600
|
2500
|
2489
|
750
|
|
260
|
622.6143791
|
2600
|
2588.340807
|
780
|
|
|
|
|
|
| Objective
Magnification: 63x |
| Value |
Leica TCS (nm) |
Zeiss LSM 310
(nm) |
Zeiss LSM 410
(nm) |
Zeiss LSM 510
(nm) |
|
10
|
75.32
|
158.73
|
158.02
|
48
|
|
20
|
111.84
|
317.46
|
316.04
|
95
|
|
30
|
148.36
|
476.19
|
474.06
|
143
|
|
40
|
184.87
|
634.92
|
632.07
|
190
|
|
50
|
221.39
|
793.65
|
790.09
|
238
|
|
60
|
257.91
|
952.38
|
948.11
|
286
|
|
70
|
294.43
|
1111.11
|
1106.13
|
333
|
|
80
|
330.95
|
1269.84
|
1264.15
|
381
|
|
90
|
367.47
|
1428.57
|
1422.17
|
429
|
|
100
|
403.98
|
1587.30
|
1580.18
|
476
|
|
110
|
440.50
|
1746.03
|
1738.20
|
524
|
|
120
|
477.02
|
1904.76
|
1896.22
|
571
|
|
130
|
513.54
|
2063.49
|
2054.24
|
619
|
|
140
|
550.06
|
2222.22
|
2212.26
|
667
|
|
150
|
586.58
|
2380.95
|
2370.28
|
714
|
|
160
|
623.09
|
2539.68
|
2528.29
|
762
|
|
170
|
659.61
|
2698.41
|
2686.31
|
810
|
|
180
|
696.13
|
2857.14
|
2844.33
|
857
|
|
190
|
732.65
|
3015.87
|
3002.35
|
905
|
|
200
|
769.17
|
3174.60
|
3160.37
|
952
|
|
210
|
805.69
|
3333.33
|
3318.39
|
1000
|
|
220
|
842.20
|
3492.06
|
3476.40
|
1048
|
|
230
|
878.72
|
3650.79
|
3634.42
|
1095
|
|
240
|
915.24
|
3809.52
|
3792.44
|
1143
|
|
250
|
951.76
|
3968.25
|
3950.46
|
1190
|
|
260
|
988.28
|
4126.98
|
4108.48
|
1238
|
|
