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Input • Reads files from Imaris, Leica, DeltaVision, Bio-Rad, SGI, TIFF series • Number of channels limited by memory only • Arbitrary selection of a cuboid image region of interest Basic Operation • 2-channel colocalization • 3-channel colocalization Histogram Generation and Selection • Creation of a 1D histogram for each channel • Creation of a 2D or 3D histogram for each set, dependent on the type of operation • Interactive selection of a rectangular Region of Interest (ROI) in 1D and 2D or 3D histograms, dependent on the type of operation • Interactive selection of a polygonal ROI in a 2D or 3D histogram, dependent on the type of operation • Interactive and calculated selection of a ROI in a 2D or 3D histogram, calculation based on linear relations between channel intensities Channel Set Definitions • Interactive selection of pairs and triplets • Automatic generation of all set permutation • Automatic generation of specific names which are used throughout the whole software • Automatic generation of specific colors which are used throughout the whole software Creation of Colocalization Images • Creates new array of colocalization images (CI) • CI can be generated as constant value, maximum value, minimum value, average value or modulus value • Display of CI and related channel images by layer or image • Display and CI can be saved as 3D images (Imaris format, or other) Reports • Generation of text reports in print or spreadsheet format • Output for layers and full channels • Free design of inclusion of values Plotting • Layer result values versus layers • Channel-set result value versus channel-sets • Layer result values versus layers versus channel-sets