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The Deconvolution Gallery. 1) DNA in the nucleus of a sea urchin cell. Image: 512 x 512 x 80. 2) The same image after a 30-iteration run using the MLE algorithm. 3) The spindle apparatus of a dividing cell shown using an antibody against tubulin (green). Chromosomes are red. Original image recorded on a Zeiss LSM 410. Image: 256 x 256 x 40. Voxel: 55 x 55 x 190 nm 4) The same image after deconvolution using eight iterations of Maximum Likelihood Estimation (MLE). Each channel has been processed independently.