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CatRetna 8Hz
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Software
: Imaris
A whole mount preparation of the cat retina has been made using immuno-fluorescence stain with antibodies against Collagen Type IV (yellow) and GFAP (cyan blue). The overall staining procedure lasted for several days allowing the antibodies penetrate the structure completely.
The shadow projection algorithm shows the following features:
shadows cast on a background layer
shadows cast onto structures in other channels
separation of the colors of the individual channel
Image courtesy of Dr. E. Rungger-Brändle and Dr. M. Messerli
References:
Messerli, J.M., van der Voort, H.T.M., Rungger-Brändle, E., and Perriard, J.-C. (1993). Three-dimensional Visualization of Multi-Channel Voxel Data. Cytometry 14, in press.
Rungger-Brändle, E., Messerli, J.M., Niemeyer, G., and Eppenberger, H.M. (1993). Confocal Microscopy and
Computer-Assisted Image Reconstruction of Astrocytes in the Mammalian Retina. Eur. J. Neuroscience in press.
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